Miltenyi MACSQuant® Tyto®

The Chair in Cellular Immunotherapy offers users from the UKW and the University of Würzburg the opportunity to use the MACSQuant® Tyto® Cell Sorter in our facility, allowing for sterile flow-based cell sorting in a closed cartridge system.

For technical assistance & booking, contact us: hudeceklab@ukw.de

Technical Specifications

OPTICS  
Laser excitation 405 nm, 100 mW
  488 nm, 100 mW
  638 nm, 100 mW
Emission detectors V1: 450/50 nm
  V2: 525/50 nm
  B1: 525/50 nm
  B2: 585/40 nm
  B3: 655–730 nm
  B4: 750 nm LP
  R1: 655–730 nm
  R2: 750 nm LP
  Backscatter and side scatter for detection of size & granularity of cells
Fluorescence performance 5 decade logarithmic scales, display in lin, log, or hlog scales

PERFORMANCE  
Purity >99% (dependent on input sample and Poisson statistics)
Cell flow rate Up to 55,000 cells/s for the MACSQuant® Tyto® Cartridge and up to 110,000 cells/s for the MACSQuant Tyto Cartridge HS
Sort rate Up to 30,000 valve actuations/s
Viability >99% for Lymphocytes (dependent on input sample and Poisson statistics)
Sorting mode Two-way sorting of sorted and non-sorted cells
Technical support Built-in live support functionality for reliable support in real time
Operating pressure <230 mbar (3 psi) with the MACSQuant® Tyto® Cartridge and 1100 mbar (<14 psi) with the MACSQuant® Tyto® Cartridge HS
Temperature control range 4–25 °C (under standard environmental conditions)
Carry-over No sample-to-sample carry-over due to single-use disposable cartridge

DATA MANAGEMENT  
Measurement Parameters Height &AMP; Area measurements for ALL Parameters
Signal processing 16-bit analog-to-digital conversion and signal processing
Operating system Core i7, 512 GB flash disk, 8 GB DDR4 RAM and Windows® 7 64-bit
Compensation Full 8×8 matrix compensation
Data files .mqd (proprietary file type); .fcs (2.0, 3.0, 3.1 compatible)
Data management  
Dimensions 965 × 585 × 280 mm3: without external monitor
Weight 62 kg (137 lbs): without external monitor
Monitors 32 ̎ display and 15.6 ̎ touchscreen (internal)
Power requirements / consumption 100-240 V, 50/60 Hz / 300 W: without external monitor
Ports 4× USB 2.0, 6× USB 3.0, 2× Ethernet, DVI-D

ENVIRONMENTAL CONDITIONS  
Operational temperature / humidity 18–25 °C / 0–85% relative humidity, non-condensating
Emission sound pressure < 70 dB(A) level at workstation <70 dB(A)

CARTRIDGE DETAILS  
Sterility Ethylene oxide-validated sterilization method to guarantee sterility of each cartridge
A-septic filters 0.1 µm hydrophobic filters at air inlet and outlet ports
Sorting channel width 25 × 50 µm
Maximum loading volume (input) 100 µL–10 mL
Maximum loading cell number 5×107 cells/mL
Cartridge alignment for laser positioning Continuous automated cartridge alignment also during the sort
Cell retrieval No cell loss - negatively sorted cells are kept in the negative collection chamber
Barcode labeling Cartridges are labeled with a barcode for easy identification and traceability

Contact us

If you are interested in joining our team, contact us via email. Please provide a Letter of Motivation stating your scientific interests, career plans and why our research is appealing to you, accompanied by your CV and two references.

Contact

Univ.-Prof. Dr. med. Michael Hudecek
Universitätsklinikum Würzburg
Department of Internal Medicine II
Oberdürrbacher Straße 6
97080 Würzburg
Germany
E-Mail: hudeceklab@ ukw.de