Publications
CRISPR 2.0 and CAR-T Cell Engineering
Petri, K.†, D’Ippolito, E., Künkele, A., Köhl, U., Busch, D. H., Einsele, H. & Hudecek, M. † (2025). Next-generation T cell immunotherapies engineered with CRISPR base and prime editing: challenges and opportunities. Nat Rev Clin Oncol, doi:10.1038/s41571-025-01072-4.
†Corresponding author- Petri, K.*, Zhang, W.*, Ma, J.*, Schmidts, A.*, Lee, H., Horng, J. E., Kim, D. Y., Kurt, I. C., Clement, K., Hsu, J. Y., Pinello, L., Maus, M. v, Joung, J. K., & Yeh, J.-R. J. (2022). CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells.
Nature Biotechnology, 40(2), 189–193. https://doi.org/10.1038/s41587-021-00901-y
- Petri, K., & Grünewald, J. (2023). An update on prime editing: recent advances and applications.
Cell and Gene Therapy Insights, 9(9), 1359–1365. https://doi.org/10.18609/cgti.2023.174
- Zhang, W., Petri, K., Ma, J., Lee, H., Tsai, C.-L., Joung, J. K., & Yeh, J.-R. J. (2023). Enhancing CRISPR prime editing by reducing misfolded pegRNA interactions.
eLife, 12. https://doi.org/10.7554/ELIFE.90948
- Grünewald, J., Miller, B. R., Szalay, R. N., Cabeceiras, P. K., Woodilla, C. J., Holtz, E. J. B., Petri, K., & Joung, J. K. (2022). Engineered CRISPR prime editors with compact, untethered reverse transcriptases.
Nature Biotechnology 2022, 1–7. https://doi.org/10.1038/s41587-022-01473-1
- Annas, G. J., Beisel, C. L., Clement, K., Crisanti, A., Francis, S., Galardini, M., Galizi, R., Grünewald, J., Immobile, G., Khalil, A. S., Müller, R., Pattanayak, V., Petri, K., Paul, L., Pinello, L., Simoni, A., Taxiarchi, C., & Joung, J. K. (2021). A Code of Ethics for Gene Drive Research.
The CRISPR Journal, 4(1), 19–24. https://doi.org/10.1089/crispr.2020.0096
- Hsu, J. Y., Grünewald, J., Szalay, R., Shih, J., Anzalone, A. v., Lam, K. C., Shen, M. W., Petri, K., Liu, D. R., Keith Joung, J., & Pinello, L. (2021). PrimeDesign software for rapid and simplified design of prime editing guide RNAs.
Nature Communications 2021 12:1, 12(1), 1–6. https://doi.org/10.1038/s41467-021-21337-7
- Petri, K., & Pattanayak, V. (2018). SHERLOCK and DETECTR Open a New Frontier in Molecular Diagnostics.
The CRISPR Journal, 1(3), 209–211. https://doi.org/10.1089/crispr.2018.29018.kpe
Assessing the Precision of CRISPR Gene Editing
- Zeng, J., Nguyen, M. A., Liu, P., da Silva, L. F., Levesque, S., Lin, L. Y., Justus, D. G., Petri, K., Clement, K., Porter, S. N., Verma, A., Neri, N. R., Rosanwo, T., Ciuculescu, M.-F., Abriss, D., Mintzer, E., Maitland, S. A., Demirci, S., Cha, H. J., … Bauer, D. E. (2024). Gene editing without ex vivo culture evades genotoxicity in human hematopoietic stem cells.
Cell Stem Cell. https://doi.org/10.1016/j.stem.2024.11.001
- Petri, K., Kim, D. Y., Sasaki, K. E., Canver, M. C., Wang, X., Shah, H., Lee, H., Horng, J. E., Clement, K., Iyer, S., Garcia, S. P., Guo, J. A., Newby, G. A., Pinello, L., Liu, D. R., Aryee, M. J., Musunuru, K., Joung, J. K., & Pattanayak, V. (2021). Global-scale CRISPR gene editor specificity profiling by ONE-seq identifies population-specific, variant off-target effects.
bioRxiv, 2021.04.05.438458. https://doi.org/10.1101/2021.04.05.438458
- Garrood, W. T., Kranjc, N., Petri, K., Kim, D. Y., Guo, J. A., Hammond, A. M., Morianou, I., Pattanayak, V., Joung, J. K., Crisanti, A., & Simoni, A. (2021). Analysis of off-target effects in CRISPR-based gene drives in the human malaria mosquito.
Proceedings of the National Academy of Sciences of the United States of America, 118(22). https://doi.org/10.1073/PNAS.200483811
- Konstantinidis, E., Molisak, A., Perrin, F., Streubel-Gallasch, L., Fayad, S., Kim, D. Y., Petri, K., Aryee, M. J., Aguilar, X., György, B., Giedraitis, V., Joung, J. K., Pattanayak, V., Essand, M., Erlandsson, A., Berezovska, O., & Ingelsson, M. (2022). CRISPR-Cas9 treatment partially restores amyloid-β 42/40 in human fibroblasts with the Alzheimer's disease PSEN 1 M146L mutation.
Molecular Therapy. Nucleic Acids, 28, 450–461. https://doi.org/10.1016/J.OMTN.2022.03.022
- Knipping, F., Osborn, M. J., Petri, K., Tolar, J., Glimm, H., von Kalle, C., Schmidt, M., & Gabriel, R. (2017). Genome-wide Specificity of Highly Efficient TALENs and CRISPR/Cas9 for T Cell Receptor Modification.
Molecular Therapy - Methods and Clinical Development, 4. https://doi.org/10.1016/j.omtm.2017.01.005
Integration Site Profiling of Viruses and Viral Vectors
- Petri, K.*, Gabriel, R.*, Agundez, L.*, Fronza, R., Afzal, S., Kaeppel, C., Linden, R. M., Henckaerts, E., & Schmidt, M. (2015). Presence of a trs-like motif promotes Rep-mediated wildtype adeno-associated virus type 2 integration.
Journal of Virology, 89(14). https://doi.org/10.1128/JVI.00426-15
- Petri, K., Fronza, R., Gabriel, R., Käppel, C., Nowrouzi, A., Linden, R. M., Henckaerts, E., & Schmidt, M. (2014). Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats.
BioTechniques, 56(5), 269–273. https://doi.org/10.2144/000114170